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Pharmacodynamic study in DIO mice Female DIO mice with a body weight approximately 22 g received tail vein injections of either saline (Saline), scExe4 donor plasmid loaded LNP only (Donor), or scExe4 donor plasmid loaded LNP plus Cas9 plasmid loaded LNP (Donor+Cas9)

[78] Infantile scurvy [79] [80] [81] [82] emerged in the late 19th century because children were fed pasteurized cow's milk, particularly in the urban upper class

Combined Mc3r and Mc4r deficiency further exacerbates the obese phenotype observed by Mc4r deficiency alone, as Mc3r deficiency enhances food efficiency and storage of fat [117]

doi: 10.1007/s12031-018-1225-9